![]() This supports the practice of testing CSF of seropositive horses suspected of having EPM. Our results demonstrate that the level of agreement is only moderate in diagnostic samples. All detect the presence of antibodies in the. EPM was scored by recording the time spent in each of 5 segments of the apparatus. Quantification of western blot results for Arc in the (b). In addition, 29% (33/112) CSF samples from seropositive horses were negative. Tests for EPM in live horses include the Western blot, indirect fluorescent antibody test (IFAT), and surface antigen-1 ELISA test (SAG-1 ELISA). Full length blot images in Supplementary Fig. The kappa statistic for the analysis in which the suspect results were reclassified as negative was 0.474. The kappa statistic for negative, positive and suspect samples was 0.469. An additional analysis was performed in which samples reported as suspect were reclassified as negative. The agreement of the paired serum and CSF results was assessed for three possible test outcomes-negative, positive or suspect. The purpose of this study was to assess the agreement of the results of 181 paired serum and CSF Western blot antibody tests on equine samples submitted to the Michigan State University Animal Health Diagnostic Laboratory. The dos and donts of Western Blot testing for EPM, and Sarcocystis sp. If the agreement between serum and CSF test results is strong, serum tests could be used to substitute for CSF tests in some cases. L.A (1994) Results of cerebrospinal fluid analysis in 119 horses testing positive to the Western blot test on both serum and CSF to equine protozoal. Sera from all challenged horses yielded positive western blot results within 56. The negative predictive value of the Western blot test is generally accepted to be high for both serum and CSF. Equine protozoal myeloencephalitis (EPM) is a serious disease of horses. It’s used to confirm or disprove the results of an earlier test for HIV or Lyme disease. Numerous serologic tests have become available during the past 2 decades to aid in the diagnosis of EPM caused by S. neurona in the blood and/or cerebrospinal fluid (CSF). The Western blot test is an antibody test that’s done on a blood sample. Veterinarians diagnose EPM by performing a neurological examination and by ordering Western blot tests for antibodies to S. Equine protozoal myeloencephalitis (EPM) is a neurological disease of equids that is caused by infection of the central nervous system with Sarcocystis neurona.
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